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課程名稱 |
基因科技學
CENE TECHNOLOGY |
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開課學期 |
96-2 |
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授課對象 |
生命科學院 生命科學系 |
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授課教師 |
蔡懷楨 |
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課號 |
MCB5009 |
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課程識別碼 |
B43 U1200 |
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班次 |
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學分 |
2 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二5,6(12:20~14:10) |
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上課地點 |
生科3A |
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備註 |
限學士班二年級以上
總人數上限：70人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/962GT |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
Tue 1:20-3:10 pm, Rm LSB 3A, (Spring, 2008)
Huai-Jen Tsai, IMCB, 3366-2487; hjtsai@ntu.edu.tw
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課程目標 |
Ch. 1 Why gene cloning and DNA analysis are important?
Ch. 2 Vehicles for gene cloning: plasmids and bacteriophages
Ch. 3 Purification of DNA from living cells
Ch. 4 Manipulation of purified DNA
Ch. 5 Introduction of DNA into living cells
Ch. 6 Cloning vectors for E. coli
Ch. 7 Cloning vectors for eukaryotes
Ch. 8 How to obtain a clone of a specific gene
Ch. 9 PCR
Ch. 10 Studying gene location and structure
Ch. 11 Studying gene expression and function
Ch. 12 Studying genomes
Ch. 13 Purification of protein from cloned genes
Ch. 14 Gene cloning and DNA analysis in medicine
Ch. 15 Gene cloning and DNA analysis in agriculture
Ch. 16 Gene cloning and DNA analysis in forensic science
Gene transfer technology: mice
Gene transfer technology: model fish
Gene transfer technology: plant
Microarray, Real-time PCR
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課程要求 |
Textbooks:
1) Brown, 2006, Gene Cloning and DNA Analysis (5th edition), Blackwell Science 2) Reece, 2003, Analysis of Genes and Genomes, John Wiley & Sons
3) Barnum, 2005, Biotechnology (2nd edition), Thomson
成績評量：期中考或（及）期末考；繳交學期報告為optional. |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
Textbooks:
1) Brown, 2006, Gene Cloning and DNA Analysis (5th edition), Blackwell Science
2) Reece, 2003, Analysis of Genes and Genomes, John Wiley & Sons
3) Barnum, 2005, Biotechnology (2nd edition), Thomson
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
期中考 |
20% |
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2. |
期末考 |
20% |
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3. |
隨堂測驗 |
20% |
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4. |
作業 |
20% |
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5. |
報告 |
20% |
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週次 |
日期 |
單元主題 |
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第1週 |
2/19 |
Ch. 1 Why gene cloning and DNA analysis are important?
Ch. 2 Vehicles for gene cloning: plasmids and bacteriophages |
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第2週 |
2/26 |
Ch. 3 Purification of DNA from living cells
Ch. 4 Manipulation of purified DNA |
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第3週 |
3/04 |
Ch. 5 Introduction of DNA into living cells |
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第4週 |
3/11 |
Ch. 6 Cloning vectors for E. coli |
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第5週 |
3/18 |
Ch. 7 Cloning vectors for eukaryotes |
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第6週 |
3/25 |
Ch. 8 How to obtain a clone of a specific gene |
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第7週 |
4/01 |
Ch. 9 PCRCh. 10 Studying gene location and structure |
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第8週 |
4/08 |
Ch. 11 Studying gene expression and function |
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第9週 |
4/15 |
Midterm Exam |
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第10週 |
4/22 |
Ch. 12 Studying genomes |
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第11週 |
4/29 |
Ch. 13 Purification of protein from cloned genes |
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第12週 |
5/06 |
Ch. 14 Gene cloning and DNA analysis in medicineCh. 15 Gene cloning and DNA analysis in agriculture (I) |
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第13週 |
5/13 |
Ch. 15 Gene cloning and DNA analysis in agriculture (II)Ch. 16 Gene cloning and DNA analysis in forensic science |
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第14週 |
5/20 |
Gene transfer technology: mice |
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第15週 |
5/27 |
Gene transfer technology: model fish |
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第16週 |
6/03 |
Gene transfer technology: plant |
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第17週 |
6/10 |
Microarray, Real-time PCR |
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第18週 |
06/17 |
Final Exam |
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